Researchers have developed a method to produce human pluripotent stem cells induced, or CSPI, which contain no foreign DNA potentially harmful.
This result is important for the use of these cells in basic research in biology and is a key step in light of CSPI produce usable medical therapy without the risk that elements inserted into their genome interfere with normal cell development.
When researchers in the early stages reprogrammed adult cells and fetal to become CSPI, they used viruses to insert transformed key genes in the cell nucleus can trigger the process of reprogramming. Jungying Yu and his colleagues now describe an alternative to this approach. They added genes to circular DNA fragments called plasmids in general independent of cellular chromosomes. Then they introduced plasmids in human foreskin cells by a process called nucléofection. The proteins expressed by genes carried by plasmids were then able to reprogram cells by CSPI.
Finally, the CSPI began to lose their plasmid over cell division and then the researchers managed to isolate that do more focused. In their article, the authors state that other teams have recently reported methods with the same objective but their process is currently the only producing CSPI completely devoid of human vectors and transgenic sequences.